原代細胞分離中，針對生殖相關細胞組織涉及到黃體細胞，黃體、成纖維細胞、上皮，子宮內膜，卵巢，子宮和羊膜間充質干細胞等等的解離通常會使用酶，如何選擇合適的酶來完成對應生殖相關細胞組織的解離，以及原代細胞采集的常規操作， 艾美捷針對生殖相關細胞相關的組織分類解離分種屬、不同的細胞、酶組合推薦，推薦濃度、培養基以及參考文獻，詳細匯總指南：
本技術指南描述了標準實驗室程序； 為建立細胞分離方案提供了一種合乎邏輯的實驗方法； 并列出了許多組織特異性參考資料，以幫助開發有效的方法。更多其他組織的解離指南，點擊查看：各組織分離指南。
| 生殖相關細胞 | |||||
| 種屬 | 種屬詳情 | 細胞名稱 | 酶組合推薦，含推薦濃度 | 培養基 | 參考文獻 |
| 牛 | Bovine | 黃體細胞 / Corpus leuteal cells | Collagenase Type 4: 420 u/ml | M-199 | Levy N, Gordin M, Mamluk R, Yanagisawa M, Smith M F, Hampton J H, Meidan R: Distinct cellular localization and regulation of endothelin-1 and endothelin-converting enzyme-1 expression in the bovine corpus luteum: implications for luteolysis, Endocrinology 142, 5254-60, 2001 |
| 牛 | Bovine, female | 黃體 / Leuteal | Collagenase Type 1: 0.2% | Ham's F-12 | Tsang PC, Poff JP, Boulton EP, Condon WA: Four-day-old bovine corpus luteum: progesterone production and identification of matrix metalloproteinase activity in vitro, Biol Reprod 53, 1160-8, 1995 |
| 牛 | Calf, mid to late gestational | 成纖維細胞 / Fibroblasts | Collagenase: 0.1% | Medium 199 | Coplen, D., Howard, P., Duckett, J., Snyder, H., and Macarak, E.: Characterization of a Fibroblast Cell From the Urinary Bladder Wall, In Vitro Cell Dev Biol 30A, 604, 1994 |
| 牛 | Bovine | 上皮，子宮內膜 / Epithelial，Endometrial | Collagenase Type 2: 0.1% | DMEM/EBSS | Munson, L., Chandler, S., and Schlafer, D.: Long-Term Culture of Bovine Trophoblastic Cells, J Tiss Cul Meth 11 (3), 123, 1988 |
| 牛 | Bovine | 內，黃體，子宮內膜，卵巢，子宮 / Interna & corpus luteum，Endometrium，Ovarian，Uterine | Pronase: 0.1% | Moscona's BSS | Marcus, G., Connor, L., Domingo, M., Tsang, B., Downey, B., and Ainsworth, L.: Enzymatic Dissociation of Ovarian and Uterine Tissues, Endocr Res 10, 151, 1984 |
| 犬/狗 | Canine | 羊膜間充質干細胞 / Amniotic membrane MSC | Trypsin: 0.25%； Collagenase Type 1: 0.2% | LG-DMEM | Park, S., Seo, M., Kim, H. and Kang, K.: Isolation and Characterization of Canine Amniotic Membrane-Derived Multipotent Stem Cells., PLoS ONE 7, e44693, 2012 |
| 雞 | Chicken, Gallus Domesticus,20-30 week | 初級卵泡 / Primary follicles | Trypsin: 0.15%； Collagenase Type 1: 0.125% | Dulbecco's phosphate buffered saline | Du Meihong, Han Haitang, Jiang Bin, Zhao Chen, Qian Changsong, Shen Haiyan, Xu Yan, Li Zandong: An efficient isolation method for domestic hen (Gallus domesticus) ovarian primary follicles, J Reprod Dev 52, 569-76, 2006 |
| 魚 | Sebastes Schlegel | 卵巢 / Ovarian | Collagenase Type 1: 500 u/ml | L-15 | Ryu, J., Kim, H., Bae, S., Jung, C. and Gong, S.: Isolation and in Vitro Culture of Primary Cell Populations Derived from Ovarian Tissues of the Rockfish, Sebastes schlegeli, Fish Aquatic Sci 19, , 2016 |
| 青蛙 | Xenopus Laevis, female | 卵母細胞 / Oocytes | Collagenase Type 1: 0.2% | Barth's solution | Nicoll, G, Jawad, A., Weymouth, R, Zhang, H. and Beg, A.: Pharmacological characterization of the excitatory ‘Cys-loop’ GABA receptor family in Caenorhabditis elegans, Br J Pharmacol 174, 781, 2017 |
| 青蛙 | Xenopus laevis | 卵母細胞 / Oocytes | Collagenase: 0.2% | Barth's solution | Chatzidaki, A., D'Oyley, J., Gill-Thind, J., Sheppard, T. and Millar, N.: The Influence of Allosteric Modulators and Transmembrane Mutations on Desensitisation and Activation of alpha7 Nicotinic Acetylcholine Receptors., Neuropharmacol 97, 75-85, 2015 |
| 青蛙 | Xenopus | 卵母細胞 / Oocytes | Collagenase Type 2: 0.2% | see reference | O'Connell, D., Mruk, K., Rocheleau, J. and Kobertz, W.: Xenopus laevis Oocytes Infected with Multi-drug-resistant Bacteria: Implications for Electrical Recordings., J Gen Physiol 138, 271-7, 2011 |
| 青蛙 | Xenopus laevis, female | 卵母細胞 / Oocytes | Collagenase: 0.2% | see reference | Mruk, K. and Kobertz, W.: Discovery of a Novel Activator of KCNQ1-KCNE1 K Channel Complexes., PLoS ONE 4, e4236, 2009 |
| 青蛙 | Xenopus laevis, female | 卵母細胞 / Oocytes | Collagenase: 0.5% | Barth's solution, CF | Cohen, S., Au, S. and Pante, N.: Microinjection of Xenopus Laevis Oocytes., J Vis Exp 24, 1106, 2009 |
| 青蛙 | Xenopus laevis, female | 卵母細胞 / Oocytes | Collagenase Type 1: 0.2% | see reference | Pannaccione Anna, Castaldo Pasqualina, Ficker Eckhard, Annunziato Lucio, Taglialatela Maurizio: Histidines 578 and 587 in the S5-S6 linker of the human Ether-a-gogo Related Gene-1 K+ channels confer sensitivity to reactive oxygen species, J Biol Chem 277, 8912-9, 2002 |
| 青蛙 | Xenopus laevis, female | 卵母細胞 / Oocytes | Collagenase Type 1: 0.2% | CF Medium | Alagem, N., Dvir, M., and Reuveny, E.: Mechanism of Ba2+ Block of a Mouse Inwardly Rectifying K+ Channel: Differential Contribution by Two Discrete Residues, J Physiol 534 (2), 381, 2001 |
| 青蛙 | Xenopus | 卵母細胞 / Oocytes | Collagenase Type 1: 1% | (see reference) | Tian, J., Kim, S., Heilig, E., and Ruderman, J.: Identification of XPR-1, A Progesterone Receptor Required for Xenopus Oocyte Activation, Proc Natl Acad Sci U S A 97, 14358, 2000 |
| 青蛙 | Xenopus laevis, female | 卵母細胞 / Oocytes | Collagenase: 0.1% | Barth's solution, CF | Karkanias, N, and Papke, R.: Subtype-Specific Effects of Lithium on Glutamate Receptor Function, J Neurophysiol 81, 1506, 1999 |
| 青蛙 | Xenopus laevis, mature female | 卵母細胞 / Oocytes | Collagenase: 0.2% | CF Medium | Moriarty, T., Gillo, B., Carty, D., Premont, R., Landau, E., Iyengar, R.: Beta gamma Subunits of GTP-Binding Proteins Inhibit Muscarinic Receptor Stimulation of Phospholipase C, Proc Natl Acad Sci U S A 85, 8865, 1988 |
| 倉鼠 | Hamster, Chinese | 子房 / Ovary | Trypsin: 5% | Dialyzed fetal calf serum, 10% and 0.5M Methotrexate | Wallis, R., and Drickamer, K.: Molecular Determinants of Oligomer Formation and Complement Fixation in Mannose-Binding Proteins, J Biol Chem 274 (6), 3580, 1999 |
| 人 | Human | 子宮內膜上皮和間質成纖維細胞 / Endometrial epithelial and stromal fibroblasts | Collagenase Type 1: 0.32%； Hyaluronidase: 62 u/ml | DMEM | Chen, J. and Roan, N.: Isolation and Culture of Human Endometrial Epithelial Cells and Stromal Fibroblasts., Bio Protoc 5, , 2015 |
| 人 | Human | 子宮內膜間質 / Endometrial stromal | Collagenase Type 3: 8 u/ml； Deoxyribonuclease I: 0.01% | DMEM/F-12 | Evans, J. and Salamonsen, L.: Decidualized Human Endometrial Stromal Cells are Sensors of Hormone Withdrawal in the Menstrual Inflammatory Cascade., Biol Reprod 90, 14, 2014 |
| 人 | Human | 內皮集落形成 / Endothelial colony forming | Collagenase Type 1: 0.1%； Deoxyribonuclease I: 0.1%； Neutral Protease: 0.075% | HBSS | Patel, J., Seppanen, E., Chong, M., Yeo, J., Teo, E., Chan, J., Fisk, N. and Khosrotehrani, K.: Prospective Surface Marker-Based Isolation and Expansion of Fetal Endothelial Colony-Forming Cells from Human Term Placenta., Stem Cells Transl Med 2, 839-47, 2013 |
| 人 | Human | 子宮上皮 / Uterine epithelial | Pancreatin: 0.34%； Hyaluronidase: 0.01%； Collagenase: 0.16% | HBSS | Patel, M., Ghosh, M., Fahey, J. and Wira, C.: Uterine Epithelial Cells Specifically Induce Interferon-Stimulated Genes in Response to Polyinosinic-Polycytidylic Acid Independently of Estradiol., PLoS ONE 7, e35654, 2012 |
| 人 | Human | 間充質干細胞 / Mesenchymal stem | Collagenase Type 1: 0.4%； Deoxyribonuclease I: 0.01% | DMEM/F12 | Shalini, V., Pratheep, S., Muhammad, A., Sharmili, V., Elizabeth, G. and Rajesh, R.: Generation and Characterisation of Human Mesenchymal Stem Cells Derived From Umbilical Cord and Placenta, Regenerative Research 1, 48, 2012 |
| 人 | Human | 間充質干細胞 / Mesenchymal stem | Collagenase Type 2: 10%； Neutral Protease: see reference | CMF-DPBS | Steigman, S. and Fauza, D.: Isolation of Mesenchymal Stem Cells from Amniotic Fluid and Placenta., Curr Protoc Stem Cell Biol Chapter 1, Unit 1E.2, 2007 |
| 人 | Human, female | 蛻膜 / Decidual | Collagenase: 0.25%； Deoxyribonuclease I: 6.25 u/ml | DMEM/F12 | Lockwood, C., Arcuri, F., Toti, P., Felice, C., Krikun, G., Guller, S., Buchwalder, L. and Schatz, F.: Tumor Necrosis Factor-Alpha and Interleukin-1 Beta Regulate Interleukin-8 Expression in Third Trimester Decidual Cells: Implications for the Genesis of Chorioamnionitis., Am J Pathol 169, 1294-302, 2006 |
| 人 | Human | 絨毛外滋養細胞和蛻膜基質細胞 / Extravillanous trophoblasts and decidual stromal | Trypsin: 0.1-0.25%； Collagenase Type 1: 0.1%； Deoxyribonuclease I: 0.02-0.05% | HBSS RPMI | Spessotto, P., Bulla, R., Danussi, C., Radillo, O., Cervi, M., Monami, G., Bossi, F., Tedesco, F., Doliana, R. and Colombatti, A.: EMILIN1 Represents a Major Stromal Element Determining Human Trophoblast Invasion of the Uterine Wall., J Cell Sci 119, 4574, 2006 |
| 人 | Human | 支持細胞 / Sertoli cells | Trypsin: 2.5%； Collagenase Type 1: 2%； Hyaluronidase: 1% | DMEM/F-12 | Teng Yan, Xue Wu-jun, Ding Xiao-ming, Feng Xin-shun, Xiang He-li, Jiang Ya-zhuo, Tian Pu-xun: Isolation and culture of adult Sertoli cells and their effects on the function of co-cultured allogeneic islets in vitro, Chin Med J (Engl) 118, 1857-62, 2005 |
| 人 | Human, female | 子宮上皮 / Uterine epithelial | Pancreatin: 0.34%； Hyaluronidase: 0.01%； Collagenase: 0.16% | HBSS | Meter, R., Wira, C. and Fahey, J.: Secretion of Monocyte Chemotactic Protein-1 by Human Uterine Epithelium Directs Monocyte Migration in Culture., Fertil Steril 84, 191, 2005 |
| 人 | Human, female, 34-51 yr | 子宮內膜上皮和基質細胞 / Endometrial epithelial and stromal cells | Collagenase Type 3: 0.03%； Deoxyribonuclease I: 0.004% | DMEM/F-12 | Chan Rachel W S, Schwab Kjiana E, Gargett Caroline E: Clonogenicity of human endometrial epithelial and stromal cells, Biol Reprod 70, 1738-50, 2004 |
產品訂購：sales@amyjet.com
郵政編碼：430070
公司地址：武漢市洪山區光谷大道35號
光谷總部國際二期時代1棟13樓
版權所有：艾美捷科技有限公司 鄂ICP備10204150號-1 鄂公網安備：42018502004523號
微信掃碼在線客服
