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worthington 生殖相關細胞組織分離指南

發布者:艾美捷科技    發布時間:2022-06-09     

原代細胞分離中,針對生殖相關細胞組織涉及到黃體細胞,黃體、成纖維細胞、上皮,子宮內膜,卵巢,子宮和羊膜間充質干細胞等等的解離通常會使用酶,如何選擇合適的酶來完成對應生殖相關細胞組織的解離,以及原代細胞采集的常規操作, 艾美捷針對生殖相關細胞相關的組織分類解離分種屬、不同的細胞、酶組合推薦,推薦濃度、培養基以及參考文獻,詳細匯總指南:生殖相關組織細胞分離指南.png


本技術指南描述了標準實驗室程序; 為建立細胞分離方案提供了一種合乎邏輯的實驗方法; 并列出了許多組織特異性參考資料,以幫助開發有效的方法。更多其他組織的解離指南,點擊查看:各組織分離指南。


Bovine黃體細胞 / Corpus leuteal cellsCollagenase Type 4: 420 u/mlM-199Levy N, Gordin M, Mamluk R, Yanagisawa M, Smith M F, Hampton J H, Meidan R: Distinct cellular localization and regulation of endothelin-1 and endothelin-converting enzyme-1 expression in the bovine corpus luteum: implications for luteolysis, Endocrinology 142, 5254-60, 2001
Bovine, female黃體 / LeutealCollagenase Type 1: 0.2%Ham's F-12Tsang PC, Poff JP, Boulton EP, Condon WA: Four-day-old bovine corpus luteum: progesterone production and identification of matrix metalloproteinase activity in vitro, Biol Reprod 53, 1160-8, 1995
Calf, mid to late gestational成纖維細胞 / FibroblastsCollagenase: 0.1%Medium 199Coplen, D., Howard, P., Duckett, J., Snyder, H., and Macarak, E.: Characterization of a Fibroblast Cell From the Urinary Bladder Wall, In Vitro Cell Dev Biol 30A, 604, 1994
Bovine上皮,子宮內膜 / Epithelial,EndometrialCollagenase Type 2: 0.1%DMEM/EBSSMunson, L., Chandler, S., and Schlafer, D.: Long-Term Culture of Bovine Trophoblastic Cells, J Tiss Cul Meth 11 (3), 123, 1988
Bovine內,黃體,子宮內膜,卵巢,子宮 / Interna & corpus luteum,Endometrium,Ovarian,UterinePronase: 0.1%Moscona's BSSMarcus, G., Connor, L., Domingo, M., Tsang, B., Downey, B., and Ainsworth, L.: Enzymatic Dissociation of Ovarian and Uterine Tissues, Endocr Res 10, 151, 1984
犬/狗Canine羊膜間充質干細胞 / Amniotic membrane MSCTrypsin: 0.25%;
Collagenase Type 1: 0.2%
LG-DMEMPark, S., Seo, M., Kim, H. and Kang, K.: Isolation and Characterization of Canine Amniotic Membrane-Derived Multipotent Stem Cells., PLoS ONE 7, e44693, 2012
Chicken, Gallus Domesticus,20-30 week初級卵泡 / Primary folliclesTrypsin: 0.15%;
Collagenase Type 1: 0.125%
Dulbecco's phosphate buffered salineDu Meihong, Han Haitang, Jiang Bin, Zhao Chen, Qian Changsong, Shen Haiyan, Xu Yan, Li Zandong: An efficient isolation method for domestic hen (Gallus domesticus) ovarian primary follicles, J Reprod Dev 52, 569-76, 2006
Sebastes Schlegel卵巢 / OvarianCollagenase Type 1: 500 u/mlL-15Ryu, J., Kim, H., Bae, S., Jung, C. and Gong, S.: Isolation and in Vitro Culture of Primary Cell Populations Derived from Ovarian Tissues of the Rockfish, Sebastes schlegeli, Fish Aquatic Sci 19, , 2016
青蛙Xenopus Laevis, female卵母細胞 / OocytesCollagenase Type 1: 0.2%Barth's solutionNicoll, G, Jawad, A., Weymouth, R, Zhang, H. and Beg, A.: Pharmacological characterization of the excitatory ‘Cys-loop’ GABA receptor family in Caenorhabditis elegans, Br J Pharmacol 174, 781, 2017
青蛙Xenopus laevis卵母細胞 / OocytesCollagenase: 0.2%Barth's solutionChatzidaki, A., D'Oyley, J., Gill-Thind, J., Sheppard, T. and Millar, N.: The Influence of Allosteric Modulators and Transmembrane Mutations on Desensitisation and Activation of alpha7 Nicotinic Acetylcholine Receptors., Neuropharmacol 97, 75-85, 2015
青蛙Xenopus卵母細胞 / OocytesCollagenase Type 2: 0.2%see referenceO'Connell, D., Mruk, K., Rocheleau, J. and Kobertz, W.: Xenopus laevis Oocytes Infected with Multi-drug-resistant Bacteria: Implications for Electrical Recordings., J Gen Physiol 138, 271-7, 2011
青蛙Xenopus laevis, female卵母細胞 / OocytesCollagenase: 0.2%see referenceMruk, K. and Kobertz, W.: Discovery of a Novel Activator of KCNQ1-KCNE1 K Channel Complexes., PLoS ONE 4, e4236, 2009
青蛙Xenopus laevis, female卵母細胞 / OocytesCollagenase: 0.5%Barth's solution, CFCohen, S., Au, S. and Pante, N.: Microinjection of Xenopus Laevis Oocytes., J Vis Exp 24, 1106, 2009
青蛙Xenopus laevis, female卵母細胞 / OocytesCollagenase Type 1: 0.2%see referencePannaccione Anna, Castaldo Pasqualina, Ficker Eckhard, Annunziato Lucio, Taglialatela Maurizio: Histidines 578 and 587 in the S5-S6 linker of the human Ether-a-gogo Related Gene-1 K+ channels confer sensitivity to reactive oxygen species, J Biol Chem 277, 8912-9, 2002
青蛙Xenopus laevis, female卵母細胞 / OocytesCollagenase Type 1: 0.2%CF MediumAlagem, N., Dvir, M., and Reuveny, E.: Mechanism of Ba2+ Block of a Mouse Inwardly Rectifying K+ Channel: Differential Contribution by Two Discrete Residues, J Physiol 534 (2), 381, 2001
青蛙Xenopus卵母細胞 / OocytesCollagenase Type 1: 1%(see reference)Tian, J., Kim, S., Heilig, E., and Ruderman, J.: Identification of XPR-1, A Progesterone Receptor Required for Xenopus Oocyte Activation, Proc Natl Acad Sci U S A 97, 14358, 2000
青蛙Xenopus laevis, female卵母細胞 / OocytesCollagenase: 0.1%Barth's solution, CFKarkanias, N, and Papke, R.: Subtype-Specific Effects of Lithium on Glutamate Receptor Function, J Neurophysiol 81, 1506, 1999
青蛙Xenopus laevis, mature female卵母細胞 / OocytesCollagenase: 0.2%CF MediumMoriarty, T., Gillo, B., Carty, D., Premont, R., Landau, E., Iyengar, R.: Beta gamma Subunits of GTP-Binding Proteins Inhibit Muscarinic Receptor Stimulation of Phospholipase C, Proc Natl Acad Sci U S A 85, 8865, 1988
倉鼠Hamster, Chinese子房 / OvaryTrypsin: 5%Dialyzed fetal calf serum, 10% and 0.5M MethotrexateWallis, R., and Drickamer, K.: Molecular Determinants of Oligomer Formation and Complement Fixation in Mannose-Binding Proteins, J Biol Chem 274 (6), 3580, 1999
Human子宮內膜上皮和間質成纖維細胞 / Endometrial epithelial and stromal fibroblastsCollagenase Type 1: 0.32%;
Hyaluronidase: 62 u/ml
DMEMChen, J. and Roan, N.: Isolation and Culture of Human Endometrial Epithelial Cells and Stromal Fibroblasts., Bio Protoc 5, , 2015
Human子宮內膜間質 / Endometrial stromalCollagenase Type 3: 8 u/ml;
Deoxyribonuclease I: 0.01%
DMEM/F-12Evans, J. and Salamonsen, L.: Decidualized Human Endometrial Stromal Cells are Sensors of Hormone Withdrawal in the Menstrual Inflammatory Cascade., Biol Reprod 90, 14, 2014
Human內皮集落形成 / Endothelial colony formingCollagenase Type 1: 0.1%;
Deoxyribonuclease I: 0.1%;
Neutral Protease: 0.075%
HBSSPatel, J., Seppanen, E., Chong, M., Yeo, J., Teo, E., Chan, J., Fisk, N. and Khosrotehrani, K.: Prospective Surface Marker-Based Isolation and Expansion of Fetal Endothelial Colony-Forming Cells from Human Term Placenta., Stem Cells Transl Med 2, 839-47, 2013
Human子宮上皮 / Uterine epithelialPancreatin: 0.34%;
Hyaluronidase: 0.01%;
Collagenase: 0.16%
HBSSPatel, M., Ghosh, M., Fahey, J. and Wira, C.: Uterine Epithelial Cells Specifically Induce Interferon-Stimulated Genes in Response to Polyinosinic-Polycytidylic Acid Independently of Estradiol., PLoS ONE 7, e35654, 2012
Human間充質干細胞 / Mesenchymal stemCollagenase Type 1: 0.4%;
Deoxyribonuclease I: 0.01%
DMEM/F12Shalini, V., Pratheep, S., Muhammad, A., Sharmili, V., Elizabeth, G. and Rajesh, R.: Generation and Characterisation of Human Mesenchymal Stem Cells Derived From Umbilical Cord and Placenta, Regenerative Research 1, 48, 2012
Human間充質干細胞 / Mesenchymal stemCollagenase Type 2: 10%;
Neutral Protease: see reference
CMF-DPBSSteigman, S. and Fauza, D.: Isolation of Mesenchymal Stem Cells from Amniotic Fluid and Placenta., Curr Protoc Stem Cell Biol Chapter 1, Unit 1E.2, 2007
Human, female蛻膜 / DecidualCollagenase: 0.25%;
Deoxyribonuclease I: 6.25 u/ml
DMEM/F12Lockwood, C., Arcuri, F., Toti, P., Felice, C., Krikun, G., Guller, S., Buchwalder, L. and Schatz, F.: Tumor Necrosis Factor-Alpha and Interleukin-1 Beta Regulate Interleukin-8 Expression in Third Trimester Decidual Cells: Implications for the Genesis of Chorioamnionitis., Am J Pathol 169, 1294-302, 2006
Human絨毛外滋養細胞和蛻膜基質細胞 / Extravillanous trophoblasts and decidual stromalTrypsin: 0.1-0.25%;
Collagenase Type 1: 0.1%;
Deoxyribonuclease I: 0.02-0.05%
HBSS RPMISpessotto, P., Bulla, R., Danussi, C., Radillo, O., Cervi, M., Monami, G., Bossi, F., Tedesco, F., Doliana, R. and Colombatti, A.: EMILIN1 Represents a Major Stromal Element Determining Human Trophoblast Invasion of the Uterine Wall., J Cell Sci 119, 4574, 2006
Human支持細胞 / Sertoli cellsTrypsin: 2.5%;
Collagenase Type 1: 2%;
Hyaluronidase: 1%
DMEM/F-12Teng Yan, Xue Wu-jun, Ding Xiao-ming, Feng Xin-shun, Xiang He-li, Jiang Ya-zhuo, Tian Pu-xun: Isolation and culture of adult Sertoli cells and their effects on the function of co-cultured allogeneic islets in vitro, Chin Med J (Engl) 118, 1857-62, 2005
Human, female子宮上皮 / Uterine epithelialPancreatin: 0.34%;
Hyaluronidase: 0.01%;
Collagenase: 0.16%
HBSSMeter, R., Wira, C. and Fahey, J.: Secretion of Monocyte Chemotactic Protein-1 by Human Uterine Epithelium Directs Monocyte Migration in Culture., Fertil Steril 84, 191, 2005
Human, female, 34-51 yr子宮內膜上皮和基質細胞 / Endometrial epithelial and stromal cellsCollagenase Type 3: 0.03%;
Deoxyribonuclease I: 0.004%
DMEM/F-12Chan Rachel W S, Schwab Kjiana E, Gargett Caroline E: Clonogenicity of human endometrial epithelial and stromal cells, Biol Reprod 70, 1738-50, 2004

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