原代細胞分離中，針對前列腺組織涉及到前列腺癌成纖維細胞，前列腺基質細胞、前列腺上皮/干細胞、頰神經節，前列腺上皮/干細胞等等的解離通常會使用酶，如何選擇合適的酶來完成對應前列腺組織的解離，以及原代細胞采集的常規操作， 艾美捷針對前列腺相關的組織分類解離分種屬、不同的細胞、酶組合推薦，推薦濃度、培養基以及參考文獻，詳細匯總指南：
本技術指南描述了標準實驗室程序； 為建立細胞分離方案提供了一種合乎邏輯的實驗方法； 并列出了許多組織特異性參考資料，以幫助開發有效的方法。更多其他組織的解離指南，點擊查看：各組織分離指南。
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| 種屬 | 種屬詳情 | 細胞名稱 | 酶組合推薦，含推薦濃度 | 培養基 | 參考文獻 |
| 人 | Human | 前列腺癌成纖維細胞 / Prostatic cancer fibroblasts | Collagenase: 225 u/ml； Hyaluronidase: 125 u/ml | RPMI 1640 | Taylor, R., Toivanen, R., Frydenberg, M., Pedersen, J., Harewood, L., Collins, A., Maitland, N. and Risbridger, G.: Human Epithelial Basal Cells are Cells of Origin of Prostate Cancer, Independent of CD133 Status., Stem Cells 30, 1087, 2012 |
| 人 | Human, 52-56 yr | 前列腺基質細胞 / Prostatic stromal cells | Collagenase Type 1: 0.2% | DMEM/F-12 | Le Hanh, Arnold Julia T, McFann Kimberly K, Blackman Marc R: DHT and testosterone, but not DHEA or E2, differentially modulate IGF-I, IGFBP-2, and IGFBP-3 in human prostatic stromal cells, Am J Physiol/Endo 290, E952-60, 2006 |
| 人 | Human | 前列腺基質細胞 / Prostate stromal cells | Collagenase Type 1: 0.1% | RPMI 1640 | Nakashiro Koh-Ichi, Hara Shingo, Shinohara Yuji, Oyasu Miho, Kawamata Hitoshi, Shintani Satoru, Hamakawa Hiroyuki, Oyasu Ryoichi: Phenotypic switch from paracrine to autocrine role of hepatocyte growth factor in an androgen-independent human prostatic carcinoma cell line, CWR22R, Am J Pathol 165, 533-40, 2004 |
| 人 | Human, fetal | 前列腺成纖維細胞 / Prostatic fibroblasts | Collagenase Type 1: 0.125% | DMEM/F12 | Levine AC, Liu XH, Greenberg PD, Eliashvili M, Schiff JD, Aaronson SA, Holland JF, Kirschenbaum A: Androgens induce the expression of vascular endothelial growth factor in human fetal prostatic fibroblasts, Endocrinology 139, 4672-8, 1998 |
| 小鼠 | Mouse, 6-8 week | 前列腺上皮/干細胞 / Prostate epithelial/stem | Collagenase Type 2: 0.5%； Trypsin: 0.05% | HBSS | Burger, P., Gupta, R., Xiong, X., Ontiveros, C., Salm, S., Moscatelli, D. and Wilson, E.: High Aldehyde Dehydrogenase Activity: A Novel Functional Marker of Murine Prostate Stem/Progenitor Cells., Stem Cells 27, 2220-8, 2009 |
| 小鼠 | Mouse, 2 week | 前列腺上皮 / Prostatic epithelial | Collagenase Type 3: 170 u/ml | DMEM | Imamov Otabek, Morani Andrea, Shim Gil-Jin, Omoto Yoko, Thulin-Andersson Christina, Warner Margaret, Gustafsson Jan-Ake: Estrogen receptor beta regulates epithelial cellular differentiation in the mouse ventral prostate, Proc Natl Acad Sci U S A 101, 9375-80, 2004 |
| 小鼠 | Mouse, male | 前列腺干細胞 / Prostatic stem | Collagenase Type 1: 170 u/ml | DMEM | Dubey P, Wu H, Reiter RE, Witte ON: Alternative pathways to prostate carcinoma activate prostate stem cell antigen expression, Cancer Res 61, 3256-61, 2001 |
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