原代細胞分離中，針對垂體組織涉及到垂體，濾泡，生長激素、垂體前葉和結節等等的解離通常會使用酶，如何選擇合適的酶來完成對應垂體組織的解離，以及原代細胞采集的常規操作， 艾美捷針對垂體相關的組織分類解離分種屬、不同的細胞、酶組合推薦，推薦濃度、培養基以及參考文獻，詳細匯總指南：
本技術指南描述了標準實驗室程序； 為建立細胞分離方案提供了一種合乎邏輯的實驗方法； 并列出了許多組織特異性參考資料，以幫助開發有效的方法。更多其他組織的解離指南，點擊查看：各組織分離指南。
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| 種屬 | 種屬詳情 | 細胞名稱 | 酶組合推薦，含推薦濃度 | 培養基 | 參考文獻 |
| 牛 | Calf, male, less then 6 months old | 垂體 / Pituitary | Collagenase: 0.3% | DMEM | Hassan, H., and Merkel, R.: Perifusion Model System to Culture Bovine Hypothalamic Slices In Series with Dispersed Anterior Pituitary Cells, In Vitro Cell Dev Biol 30A, 435, 1994 |
| 牛 | Bovine | 垂體 / Pituitary | Collagenase: 0.1% | EBSS, CMF | Mason, W. and Ingram, C.: Techniques for Studying the Role of Electrical Activity in Control of Secretion by Normal Anterior Pituitary Cells, Vol. 124,, , 207, 1986 |
| 牛 | Bovine | 濾泡，垂體前葉和結節 / Follicular, anterior pituitary and pars tuberalis | Deoxyribonuclease I: 200 /ml | HBSS, CMF | Ferrara, N., Goldsmith, P., Fujii, D., and Weiner, R.: Culture and Characterization of Follicular Cells of the Bovine Anterior Pituitary and Pars Tuberalis, Vol. 124,, , 245, 1986 |
| 牛 | Calf, male, 1-6 week old | 垂體 / Pitituary | Hyaluronidase: 0.1% | DMEM | Ridgway, E., Klibanski, A., Marorana, M., Milbury, P., Kieffer, J., and Chin, W.: The Effect of Somatostatin on the Release of Thyrotropin and its Subunits from Bovine Anterior Pitituary Cells in Vitro, Endocrinology 112 (6), 1937, 1983 |
| 小鼠 | Mouse | 垂體 / Pituitary | Collagenase Type 2: 0.5%； Deoxyribonuclease I: 0.005% | HBSS | Perez Millain, M., Brinkmeier, M., Mortensen, A. and Camper, S.: PROP1 Triggers Epithelial-Mesenchymal Transition-Like Process in Pituitary Stem Cells., Elife 5, , 2016 |
| 小鼠 | Mouse | 垂體 / Pituitary | Collagenase Type 1: 1,000 u/ml； Soybean Trypsin Inhibitor: 0.01%； Hyaluronidase: 0.1%； Deoxyribonuclease I: 0.001% | DMEM | Pyczek, J., Buslei, R., Schult, D., Holsken, A., Buchfelder, M., Hess, I., Hahn, H. and Uhmann, A.: Hedgehog Signaling Activation Induces Stem Cell Proliferation and Hormone Release in the Adult Pituitary Gland., Sci Rep 6, 24928, 2016 |
| 小鼠 | Mouse, male | 垂體 / Pituitary | Collagenase: 0.4%； Hyaluronidase: 0.1%； Trypsin: 0.3% | DMEM/Han's F12 | Steveson Tami C, Ciccotosto Giuseppe D, Ma Xin-Ming, Mueller Gregory P, Mains Richard E, Eipper Betty A: Menkes protein contributes to the function of peptidylglycine alpha-amidating monooxygenase, Endocrinology 144, 188-200, 2003 |
| 綿羊 | Ovine, adult | 生長激素 / Somatotropes | Collagenase Type 1: 0.3%； Hyaluronidase: | Medium 199 | Xu Ruwei, Wang Qinling, Yan Ming, Hernandez Maria, Gong Changhong, Boon WahChin, Murata Yoko, Ueta Yoichi, Chen Chen: Orexin-A augments voltage-gated Ca2+ currents and synergistically increases growth hormone (GH) secretion with GH-releasing hormone in primary cultured ovine somatotropes, Endocrinology 143, 4609-19, 2002 |
| 大鼠 | Rat, male, 12-15 week | 垂體 / Pituitary | Collagenase Type 2: 0.4%； Deoxyribonuclease I: 0.04% | DMEM | Akieda-Asai, S., Zaima, N., Ikegami, K., Kahyo, T, Yao, I., Hatanaka, T., Iemura, S., Sugiyama, R., Yokozeki, T., Eishi, Y., Koike, M., Ikeda, K., Chiba, T., Yamaza, H., Shimokawa, I., Song, S., Matsuno, A., Mizutani, A., Sawabe, M. Chao, N., Tanaka, M., : SIRT1 Regulates Thyroid-Stimulating Hormone Release by Enhancing PIP5K gamma Activity through Deacetylation of Specific Lysine Residues in Mammals., PLoS ONE 5, e11755, 2010 |
| 大鼠 | Rat, Wistar, male, 2 month old | 垂體 / Pituitary | Trypsin: 0.1% | DMEM | Zhou, X., De Schepper, J., De Craemer, D., Delhase, M., Gys, G., Smitz, J., and Hooghe-Peters, E.: Pituitary Growth Hormone Release and Gene Expression in Cafeteria-Diet-Induced Obese Rats, J Endocrinol 159, 165, 1998 |
| 大鼠 | Rat, SD, female, 200 - 250 g | 垂體前葉 / Anterior pituitary gland | Trypsin: 0.1% | EBSS, CMF | D'Emden, M. and Wark, J.: Culture Requirements for Optimal Expression of 1,25-Dihydroxyvitamin D3-Enhanced Thyrotropin Secretion, In Vitro Cell Dev Biol 27, 197, 1991 |
| 大鼠 | Rat | 垂體 / Pituitary | Trypsin: 0.3 % | HEPES | Wilfinger, W., Larsen, W., Downs, T., and Wilbur, D.: An In Vitro Model for Studies of Intercellular Communication in Cultured Rat Anterior Pituitary Cells, Tissue Cell 16 (4), 483, 1984 |
| 大鼠 | Rat, SD, male, 250-450 g | 垂體前葉 / Anterior pituitary | Trypsin: 0.25% | Krebs | Portanova, R., Smith, D., and Sayers, G.: A Trypsin for the Preparation of Isolated Rat Anterior Pituitary, Proc Soc Exp Biol Med 133, 573, 1970 |
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