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worthington 胸腺組織分離指南

發布者:艾美捷科技    發布時間:2022-06-10     
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原代細胞分離中,針對胸腺涉及到樹突狀,胸腺基質細胞、脂肪干細胞、基質細胞,上皮,胸腺等等的解離通常會使用酶,如何選擇合適的酶來完成對應胸腺的解離,以及原代細胞采集的常規操作, 艾美捷針對胸腺相關的組織分類解離分種屬、不同的細胞、酶組合推薦,推薦濃度、培養基以及參考文獻,詳細匯總指南:胸腺組織分離指南.png

 

本技術指南描述了標準實驗室程序; 為建立細胞分離方案提供了一種合乎邏輯的實驗方法; 并列出了許多組織特異性參考資料,以幫助開發有效的方法。更多其他組織的解離指南,點擊查看:各組織分離指南。

 

胸腺
種屬種屬詳情細胞名稱酶組合推薦,含推薦濃度培養基參考文獻
Human樹突狀 / DentriticCollagenase Type 2: 0.1%;
Deoxyribonuclease I: 0.002%
RPMI 1640Vandenabeele S, Hochrein H, Mavaddat N, Winkel K, Shortman K: Human thymus contains 2 distinct dendritic cell populations, Blood 97, 1733-41, 2001
小鼠Mouse胸腺基質細胞 / Thymic stromalCollagenase Type 4: 0.02%;
Neutral Protease: 0.02%;
Deoxyribonuclease I: 0.0025%
RPMI-1640Rode, I. and Boehm, T.: Regenerative Capacity of Adult Cortical Thymic Epithelial Cells., Proc Natl Acad Sci U S A 109, 3463-8, 2012
小鼠Mouse基質細胞 / StromalCollagenase Type 3: 0.2%;
Hyaluronidase: 0.1%
RPMI 1640Phillips, Joy, Brondstetter, T., English, C., Lee, H., Virts, E, and Thoman, M.: IL-7 Gene Therapy in Aging Restores Early thymopoiesis without Reversing Involution, J Immunol , 4869, 2004
小鼠Mouse樹突狀 / DentriticCollagenase Type 3: 0.1%;
Deoxyribonuclease I: 325 u/ml
RPMI 1640Schiavoni, F, Mattei, F, Sestili, P, Borghi, P, Venditti, M, Morse, H, Belardelli, F, and Gabrieli, L: ICSBP is Essential for the Development of Mouse Type I Interferon-producing Cells and for the Generation and Activation of CD8a+ Dendritic Cells, J Exp Med 196, 1415, 2002
小鼠Mouse, 3-6 week胸腺 / ThymicCollagenase Type 3: 100-400 u/mlHBSSSmith KM, Olson DC, Hirose R, Hanahan D: Pancreatic gene expression in rare cells of thymic medulla: evidence for functional contribution to T cell tolerance, Int Immunol 9, 1355-65, 1997
小鼠Mouse (BALB/c, C3H, C57BL/6), 1-28 day上皮,胸腺 / Epithelial, thymusNeutral Protease: 1.5 痢/mlDMEMRopke, C., van Deurs, B., and Petersen, O.: Short-term Cultivation of Murine Thymic Epithelial Cells in a Serum-Free Medium, In Vitro Cell Dev Biol 26, 671, 1990
小鼠Mouse, C3H, 16 wk old, female上皮 / EpithelialCollagenase Type 3: 0.1%DMEMEhmann, U., Shiurba, R., and Peterson, W.: Long-Term Proliferation of Mouse Thymic Epithelial Cells in Culture, In Vitro Cell Dev Biol 22 (12), 738, 1986
小鼠Mouse, Swiss, 6 wk胸腺 / ThymusCollagenase Type 3: 150 u/mlDMEMJones, K. and Pierre, R.: Analysis of Cellular Heterogeneity in Mouse Thymus Cultures, In Vitro 17, 431, 1981
大鼠Rat, postnatal胸腺 / ThymicTrypsin: 0.05%HBSSBonfanti, P., Claudinot, S., Amici, A., Farley, A., Blackburn, C. and Barrandon, Y.: Microenvironmental Reprogramming of Thymic Epithelial Cells to Skin Multipotent Stem Cells., Nature 466, 978, 2010
大鼠Rat胸腺上皮 / Thymic epithelialCollagenase Type 3: 0.1%DMEMMasuda, A and Matsuyama, M: Epithelial Cell Lines From Rat Thymoma and Rat Thymus,Cell & Tissue Culture: Laboratory Procedures Vol. 2,Doyle, A., Griffiths, J., and Newell, D., John Wiley and Sons, Ltd., 21C:4.1, 1995
大鼠Rat (ACI/NMs X BUF/Mna) F1, male, 28 months ;Rat, (ACI/MNs) male, 8 weeks (also bovine, adult)上皮 / EpthelialCollagenase Type 3: 0.1%Eagle's MEM Serum-freeMasuda, A., Ohtsuka, K., and Matsuyama, M.: Establishment of Functional Epithelial Cell Lines from a Rat Thyoma and a Rat Thymus, In Vitro Cell Dev Biol 26, 713, 1990


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