原代細胞分離中,針對扁桃體涉及到扁桃體,扁桃體單核細胞等等的解離通常會使用酶,如何選擇合適的酶來完成對應扁桃體的解離,以及原代細胞采集的常規操作, 艾美捷針對扁桃體相關的組織分類解離分種屬、不同的細胞、酶組合推薦,推薦濃度、培養基以及參考文獻,詳細匯總指南:
本技術指南描述了標準實驗室程序; 為建立細胞分離方案提供了一種合乎邏輯的實驗方法; 并列出了許多組織特異性參考資料,以幫助開發有效的方法。更多其他組織的解離指南,點擊查看:各組織分離指南。
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種屬 | 種屬詳情 | 細胞名稱 | 酶組合推薦,含推薦濃度 | 培養基 | 參考文獻 |
人 | Human | 扁桃體 / Tonsillar | Collagenase Type 1: 0.2% | see reference | Muto, M., Manfroi, B., Suzuki, H., Joh, K., Nagai, M., Wakai, S., Righini, C. et al: Toll-Like Receptor 9 Stimulation Induces Aberrant Expression of a Proliferation-Inducing Ligand by Tonsillar Germinal Center B Cells in IgA Nephropathy., J Am Soc Nephrol 28, 1227-1238, 2017 |
人 | Human | 扁桃體 / Tonsillar | Collagenase Type 1: 0.1% | PBS | Schmidt, S., Schenkova, K., Adam, T., Erikson, E., Lehmann-Koch, J., Sertel, S., Verhasselt, B., Fackler, O., Lasitschka, F. and Keppler, O.: SAMHD1's Protein Expression Profile in Humans., J Leukoc Biol 98, 5-14, 2015 |
人 | Human | 扁桃體 / Tonsillar | Collagenase Type 4: 0.1% | Bambanker | Sato, D., Suzuki, Y., Kano, T., Suzuki, H., Matsuoka, J., Yokoi, H., Horikoshi, S., Ikeda, K. and Tomino, Y.: Tonsillar TLR9 Expression and Efficacy of Tonsillectomy with Steroid Pulse Therapy in IgA Nephropathy Patients., Nephrol Dial Transplant 27, 1090-7, 2012 |
人 | Human, female, 25-45 year | 扁桃體單核細胞 / Tonsillar mononuclear cells | Collagenase Type 1: 210 u/ml; Deoxyribonuclease I: 90 u/ml | RPMI | Grammer Amrie C, Slota Rebecca, Fischer Randy, Gur Hanan, Girschick Hermann, Yarboro Cheryl, Illei Gabor G, Lipsky PeterE: Abnormal germinal center reactions in systemic lupus erythematosus demonstrated by blockade of CD154-CD40 interactions, J Clin Invest 112, 1506-20, 2003 |
人 | Human | 扁桃體單核細胞 / Tonsillar mononuclear cells | Collagenase Type 1: 210 u/ml; Deoxyribonuclease I: 90 u/ml | RPMI | Grammer AC, McFarland RD, Heaney J, Darnell BF, Lipsky PE: Expression, regulation, and function of B cell-expressed CD154 in germinal centers, J Immunol 163, 4150-9, 1999 |
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